Sepiapterin reductase is an enzyme that in humans is encoded by the SPR gene. This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A human partial recombinant protein corresponding to amino acids V36-K261 was used as the immunogen for the SPR antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse
Reactivity
Human, Mouse
Recombinant
No
Antigen
SPR
Uniprot
P35270
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the SPR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
ELISA, WB
Dilution
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein) (BSA-free format available)