Src kinase-associated phosphoprotein 2 is an enzyme that in humans is encoded by the SKAP2 gene. The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids E124-E208) was used as the immunogen for the SKAP2 antibody.
Isotype
Rabbit IgG
Reactivity
Mouse, Human
Recombinant
No
Antigen
SCAP2
Uniprot
O75563
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the SKAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
Direct ELISA, FACS, WB
Dilution
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml