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Home / Primary Antibodies / Polyclonal Antibodies

SF1 Antibody / Splicing factor 1

SKU : BHA17109096

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NSJ Bioreagents

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Mfr.No.	RQ4231
Clonality	Polyclonal
Description	Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	A recombinant human protein corresponding to amino acids R160-Q266 was used as the immunogen for the SF1 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Human, Mouse, Rat
Recombinant	No
Subcellular Location	Nuclear

Antigen	SF1
Uniprot	Q15637

Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the SF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	ELISA, FACS, ICC, IF, IHC-P, WB
Dilution	Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Product Manual	Data Sheet

No Description Available.

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SKU	Supplier No.	Size	Your price	Quantity
BHA17109096	RQ4231	100ug	$439		Add to Cart

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