Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
RFC1
Uniprot
P35251
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the RFC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml