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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-KDEL Polyclonal (SPC-109)

BHA11906384
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Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Antigen KDEL
Target KDEL
Research Area Cell Signaling, Tags and Cell Markers, Organelle Markers, Chaperone Proteins, Protein Trafficking
Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Shipping Temperature Blue Ice or 4°C
Specificity Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, ICC/IF
Description WB ; ICC ; IF ; IHC
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610.

2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906384-1 SPC-109B Unconjugated 200 ug $334
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BHA11906384-2 SPC-109B-A390 ATTO 390 200 ug $438
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BHA11906384-3 SPC-109B-A488 ATTO 488 200 ug $435
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BHA11906384-4 SPC-109B-A594 ATTO 594 200 ug $435
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BHA11906384-5 SPC-109B-APC APC 200 ug $434
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BHA11906384-6 SPC-109B-BI Biotin 200 ug $423
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BHA11906384-7 SPC-109B-FITC FITC 200 ug $415
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BHA11906384-8 SPC-109B-HRP HRP 200 ug $408
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BHA11906384-9 SPC-109B-PCP PerCP 200 ug $434
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BHA11906384-10 SPC-109B-RPE RPE 200 ug $425
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BHA11906384-11 SPC-109S Unconjugated 12 ug $45
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