SOD2 (Mn)

Mfr.No.	SPC-118, SPC-118D, SPC-118D-A390, SPC-118D-A488, SPC-118D-A594, SPC-118D-APC, SPC-118D-BI, SPC-118D-FITC, SPC-118D-HRP, SPC-118D-PCP, SPC-118D-RPE, SPC-118S
Immunogen Species	Human
Alternative Names	Manganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody
Categories	Antibodies
Cellular Localization	Mitochondrion, Mitochondrion Matrix
Certificate Of Analysis	0.2 ug/ml of SPC-118 was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human SOD (Mn) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Human Mn SOD, Homo Sapiens (Human)
Immunogen Region	Recombinant Human Mn SOD Protein
Launch Date	2007/06/01
Net Weight (g)	0.1
Reactivity	Guinea Pig, African clawed frog, Wood Frog, Dog, Pig, Rat, Frog, Sheep, Mouse, Human, Gerbil, Monkey, Bovine, Rabbit, Hamster, Chicken
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Rat, Mouse, Bovine, Dog, Chicken, Gerbil, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus, Frog, Human

Antigen	SOD2
Swissprot	P04179
Accession Number	NP_000627.2
Gene Id	6648
Target	SOD2
Research Area	Cancer, Oxidative Stress, Cell Signaling, Neurodegeneration, Cardiovascular System, Neuroscience

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~25kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB
Description	WB ; IHC ; IP ; ELISA
Dilution	WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906523-1	SPC-118D	Unconjugated	100 ug	$352	Add to Cart
BHA11906523-2	SPC-118D-A390	ATTO 390	100 ug	$407	Add to Cart
BHA11906523-3	SPC-118D-A488	ATTO 488	100 ug	$406	Add to Cart
BHA11906523-4	SPC-118D-A594	ATTO 594	100 ug	$406	Add to Cart
BHA11906523-5	SPC-118D-APC	APC	100 ug	$405	Add to Cart
BHA11906523-6	SPC-118D-BI	Biotin	100 ug	$402	Add to Cart
BHA11906523-7	SPC-118D-FITC	FITC	100 ug	$397	Add to Cart
BHA11906523-8	SPC-118D-HRP	HRP	100 ug	$393	Add to Cart
BHA11906523-9	SPC-118D-PCP	PerCP	100 ug	$405	Add to Cart
BHA11906523-10	SPC-118D-RPE	RPE	100 ug	$403	Add to Cart
BHA11906523-11	SPC-118S	Unconjugated	12 ug	$45	Add to Cart

Rabbit Anti-Human SOD2 (Mn) Polyclonal (SPC-118)