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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Human Rab4 Polyclonal (SPC-141)

BHA11906662
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Antigen Rab4
Swissprot P20338
Accession Number NP_004569.2
Gene Id 5867
Target Rab4
Research Area Cell Signaling, Tags and Cell Markers, Organelle Markers, Cell Markers, Neuron Markers, Presynaptic Markers, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:150); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4).
In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~26kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, ICC/IF
Description WB ; IHC ; ICC ; IF
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:150); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7.

2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208.

3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412.

4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117.

5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886.

6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507.

7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906662-1 SPC-141D Unconjugated 100 ul $289
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BHA11906662-2 SPC-141D-A390 ATTO 390 100 ul $344
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BHA11906662-3 SPC-141D-A488 ATTO 488 100 ul $343
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BHA11906662-4 SPC-141D-A594 ATTO 594 100 ul $343
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BHA11906662-5 SPC-141D-APC APC 100 ul $341
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BHA11906662-6 SPC-141D-BI Biotin 100 ul $338
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BHA11906662-7 SPC-141D-FITC FITC 100 ul $334
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BHA11906662-8 SPC-141D-HRP HRP 100 ul $328
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BHA11906662-9 SPC-141D-PCP PerCP 100 ul $341
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BHA11906662-10 SPC-141D-RPE RPE 100 ul $339
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BHA11906662-11 SPC-141S Unconjugated 12 ul $45
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