JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....
Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Human HSP70 Acetyl Lys77 Polyclonal (SPC-743)

BHA11909933
Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
Request More Infos
Order Now

Credit card payments now incur a 3% fee.

Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
Ask Merchant
Request More Infos
Sample Request
Antigen HSP70 Acetyl Lys77
Swissprot P0DMV8/P0DMV9
Accession Number NP_005336.3
Gene Id 3303
Target HSP70 Acetyl Lys77
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 0.5mg/mL
Dilutions WB (1:1000); ELISA (1:1000); ICC/IF (1:60); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Shipping Temperature Blue Ice or 4°C
Specificity Binds to acetylated lysine 77 on HSP70. Detects 70 kDa band.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, WB, ICC/IF
Description WB ; ELISA ; ICC ; IF
Dilution WB (1:1000); ELISA (1:1000); ICC/IF (1:60); IHC (1:50); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050.

2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17.

3. Rothman J. (1989) Cell 59: 591-601.

4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.

5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.

6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.

7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424.

8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879.

9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239.

10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377.

11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248.

12. Dressel et al. (2000) J. Immunol. 164: 2362-2371.

13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555.

14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11909933-1 SPC-743D Unconjugated 100 ul $417
Add to Cart
BHA11909933-2 SPC-743D-A390 ATTO 390 100 ul $472
Add to Cart
BHA11909933-3 SPC-743D-A488 ATTO 488 100 ul $471
Add to Cart
BHA11909933-4 SPC-743D-A594 ATTO 594 100 ul $471
Add to Cart
BHA11909933-5 SPC-743D-APC APC 100 ul $470
Add to Cart
BHA11909933-6 SPC-743D-BI Biotin 100 ul $467
Add to Cart
BHA11909933-7 SPC-743D-FITC FITC 100 ul $462
Add to Cart
BHA11909933-8 SPC-743D-HRP HRP 100 ul $458
Add to Cart
BHA11909933-9 SPC-743D-PCP PerCP 100 ul $470
Add to Cart
BHA11909933-10 SPC-743D-RPE RPE 100 ul $468
Add to Cart
BHA11909933-11 SPC-743S Unconjugated 12 ul $45
Add to Cart