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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Human alpha B Crystallin Polyclonal (SPC-126)

BHA11906622
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Email
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Antigen Alpha B Crystallin
Swissprot P02511
Accession Number NP_001876.1
Gene Id 1410
Target Alpha B Crystallin
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Neuroscience, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:5000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Purification Affinity Purified
Scientific Background The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~22kDa. Does not cross-react with alphaA-crystallin.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, ICC/IF
Description WB ; IHC ; ICC ; IF
Dilution WB (1:5000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.

2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453.

3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490

4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.

5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.

6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.

7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215.

8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.

9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906622-1 SPC-126B Unconjugated 200 ul $289
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BHA11906622-2 SPC-126B-A390 ATTO 390 200 ul $393
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BHA11906622-3 SPC-126B-A488 ATTO 488 200 ul $390
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BHA11906622-4 SPC-126B-A594 ATTO 594 200 ul $390
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BHA11906622-5 SPC-126B-APC APC 200 ul $389
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BHA11906622-6 SPC-126B-BI Biotin 200 ul $379
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BHA11906622-7 SPC-126B-FITC FITC 200 ul $370
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BHA11906622-8 SPC-126B-HRP HRP 200 ul $363
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BHA11906622-9 SPC-126B-PCP PerCP 200 ul $389
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BHA11906622-10 SPC-126B-RPE RPE 200 ul $381
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BHA11906622-11 SPC-126S Unconjugated 12 ul $45
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