PRDX3 Antibody / Peroxiredoxin 3

Mfr.No.	R31762
Clonality	Polyclonal
Description	Peroxiredoxin 3, also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human protein shares 86% amino acid sequence similarity with mouse, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of Peroxiredoxin 3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that Peroxiredoxin 3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Human partial recombinant protein (AA 110-256) was used as the immunogen for this PRDX3 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Rat, Mouse, Human
Recombinant	No

Antigen	PRDX3
Gene Id	10935

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the PRDX3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	FACS, IF, ICC, IHC-P, WB
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells