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Home / Primary Antibodies / Polyclonal Antibodies

POLR2A Antibody / RNA polymerase II subunit RPB1

SKU : BHA17110320

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NSJ Bioreagents

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Mfr.No.	RQ5679
Clonality	Polyclonal
Description	DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Recombinant human protein (amino acids D10-E321) was used as the immunogen for the POLR2A antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat, Monkey
Reactivity	Rat, Monkey, Mouse, Human
Recombinant	No
Subcellular Location	Nuclear

Antigen	POLR2A
Uniprot	P24928

Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format	Antigen affinity purified
Purification	Affinity purified
Storage	After reconstitution, the POLR2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	Direct ELISA, FACS, IF, IHC-P, WB
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Product Manual	Data Sheet

No Description Available.

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SKU	Supplier No.	Size	Your price	Quantity
BHA17110320	RQ5679	100ug	$439		Add to Cart

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