DNA polymerase alpha subunit 2/B is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids N267-P579) was used as the immunogen for the POLA2 antibody.
Isotype
Rabbit IgG
Reactivity
Human
Recombinant
No
Antigen
POLA2
Uniprot
Q14181
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the POLA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
Direct ELISA, FACS, WB
Dilution
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Reviews of POLA2 Antibody / DNA polymerase alpha subunit B