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Home / Primary Antibodies / Polyclonal Antibodies

Phospho-NANOG Antibody (pS285)

SKU : BHA17101288

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NSJ Bioreagents

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Mfr.No.	F48602, F48602-0.08ML, F48602-0.4ML
Clonality	Polyclonal
Description	NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-(CG)(GA)(CG)C(GC)ATTAN(GC)-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Formulation	In 1X PBS, pH 7.4, with 0.09% sodium azide
Host	Rabbit
Immunogen Region	This phospho-NANOG antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS285 of human Nanog.
Isotype	Ig
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No

Antigen	Phospho-NANOG
Uniprot	Q9H9S0

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	Aliquot the phospho-NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Applications	DB, ELISA
Dilution	Dot blot: 1:500

Product Manual	Data Sheet

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