Peroxiredoxin 5 Antibody

Mfr.No.	R32051
Clonality	Polyclonal
Description	PRDX5 (Peroxiredoxin 5) also known as PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Amino acids 66-198 of human Peroxiredoxin 5 were used as the immunogen for the Peroxiredoxin 5 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Rat, Mouse, Human
Recombinant	No
Subcellular Location	Cytoplasmic (mitochondrial)

Antigen	Peroxiredoxin 5
Uniprot	P30044

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the Peroxiredoxin 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	FACS, IF, ICC, IHC-P, WB
Dilution	Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells