Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene. This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids S87-T211) was used as the immunogen for the PDGF alpha antibody.
Isotype
Rabbit IgG
Reactivity
Rat, Human, Mouse
Recombinant
No
Antigen
PDGFA
Uniprot
P04085
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the PDGF alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
Direct ELISA, IF, WB
Dilution
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Reviews of PDGF alpha Antibody / PDGFA / Platelet Derived Growth Factor A