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Home / Primary Antibodies / Polyclonal Antibodies

PAD4 Antibody / Protein-arginine deiminase type-4 / PADI4

SKU : BHA17104631

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Mfr.No.	R31153
Clonality	Polyclonal
Description	Peptidylarginine Deiminase, Type IV is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that the protein specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PAD4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PAD4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that the protein could repress hormone receptor-mediated gene induction. It was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	An amino acid sequence from the middle region of human PAD4 (ASPRSCYKLFQEQQNEGHGEA) was used as the immunogen for this PAD4 antibody.
Isotype	IgG
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No

Antigen	PAD4
Uniprot	Q9UM07

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the PAD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB
Dilution	Western blot: 0.5-1ug/ml

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17104631-1	R31153	100ug	$439		Add to Cart

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