Protein O-GlcNAcase (OGA) is an enzyme with systematic name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids KVYTIRPYFPKDEASVYKICRE from the human protein were used as the immunogen for the O-GlcNAcase antibody.
Isotype
Rabbit IgG
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the O-GlcNAcase antibody should be determined by the researcher.
Uniprot
O60502
Format
Antigen affinity purified
Purity
Affinity purified
Storage
After reconstitution, the O-GlcNAcase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IF, FACS
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells