This gene encodes a member of the nucleoporin family that shares 87% sequence identity with rat nucleoporin p58. The protein is localized to the nuclear rim and is a component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids R235-E438) was used as the immunogen for the NUP58 antibody.
Isotype
Rabbit IgG
Reactivity
Rat, Human
Recombinant
No
Antigen
NUP58
Uniprot
Q9BVL2
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the NUP58 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
Direct ELISA, FACS, WB
Dilution
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Reviews of NUP58 Antibody / 58 kDa nucleoporin / Nucleoporin-like protein 1