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Home / Primary Antibodies / Polyclonal Antibodies

NOXA1 Antibody / NADPH oxidase activator 1

SKU : BHA17104542

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NSJ Bioreagents

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Mfr.No.	R31030
Clonality	Polyclonal
Description	NADPH oxidase activator 1, also called NOX Activator 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the gene to chromosome 9q34.3 based on an alignment of the protein sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, but not GDP-bound RAC1. It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of NOXA1 was required for these interactions. Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	An amino acid sequence from the middle region of human NADPH oxidase activator 1 (RQVPRGEVFRPHRWHLKHLE) was used as the immunogen for this NOXA1 antibody.
Isotype	IgG
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No

Antigen	NOXA1
Uniprot	Q86UR1

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the NOXA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB
Dilution	Western blot: 0.5-1ug/ml

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17104542-1	R31030	100ug	$439		Add to Cart

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