Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1 (Na-K-Cl Cotransporter 1), is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the N-terminus of human NKCC1 (RIDHYRHTAAQLGEKLLRP) was used as the immunogen for this NKCC1 antibody (100% homologous in human, mouse and rat).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Rat, Human, Mouse
Recombinant
No
Antigen
NKCC1
Uniprot
P55011
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the NKCC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB, IHC-F
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml