CISD1, also called MitoNEET, is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity. This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active (2Fe-2S) cluster. The encoded protein has been localized to the outer membrane of mitochondria. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids Y28-E93) was used as the immunogen for the MitoNEET antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Subcellular Location
Cytoplasmic
Antigen
CISD1
Uniprot
Q9NZ45
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the MitoNEET antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, FACS, WB, Direct ELISA
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml