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Home / Primary Antibodies / Polyclonal Antibodies

Milk fat globule 1 Antibody / Mfg-e8 / Lactadherin

SKU : BHA17111062

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NSJ Bioreagents

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Mfr.No.	RQ6427
Clonality	Polyclonal
Description	MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Recombinant mouse protein (amino acids A23-C463) was used as the immunogen for the Milk fat globule 1 antibody.
Isotype	IgG
Predicted Reactivity	Mouse, Rat
Reactivity	Rat, Mouse
Recombinant	No

Antigen	Milk fat globule 1
Uniprot	P21956

Buffer	Lyophilized from 1X PBS with 2% Trehalose
Format	Purified
Purification	Antigen affinity purified
Storage	After reconstitution, the Milk fat globule 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	IHC-P, FACS, WB, Direct ELISA
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17111062	RQ6427	100ug	$439		Add to Cart

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