LFA-1 Antibody / CD18 / ITGB2

Mfr.No.	R31562
Clonality	Polyclonal
Description	Lymphocyte function associated antigen 1, also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. It is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated LFA-1/CD18. The expression of LFA-1 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of LFA-1 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although the protein is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Human partial recombinant protein (AA 404-769) was used as the immunogen for this LFA-1 antibody.
Isotype	IgG
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No

Antigen	LFA-1
Gene Id	3689

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the LFA-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	FACS, IHC-P, IF, WB, ICC
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry: 1-2ug/ml, Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-3ug/million cells