Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Host
Rabbit
Immunogen Region
Amino acids QEQKSAKSSRLPDIFEAQIAGLR from the human protein were used as the immunogen for the Keratin 7 antibody.
Isotype
Rabbit IgG
Reactivity
Human
Recombinant
No
Antigen
KRT7
Uniprot
P08729
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the Keratin 7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Reviews of Keratin 7 Antibody / Cytokeratin 7 / KRT7