Kelch repeat and BTB domain containing 2 (KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85 alpha-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85 alpha protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling. However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization. In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity and insulin sensitivity.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids QYDLELDRWSLRQHISERVLWDLGRDFR from the human protein were used as the immunogen for the KBTBD2 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Antigen
KBTBD2
Uniprot
Q8IY47
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the KBTBD2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IF, ICC, WB, FACS
Dilution
Western blot: 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells