Interferon regulatory factor 1, also known as MAR is a protein that in humans is encoded by the IRF1 gene. The IRF1 gene is mapped to chromosome 5q31.1 by pulsed-field gel electrophoresis. IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids M170-L315) was used as the immunogen for the IRF-1 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Rat, Mouse, Human
Recombinant
No
Subcellular Location
Nuclear, cytoplasmic
Antigen
IRF1
Uniprot
P10914
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the IRF-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
Direct ELISA, IF, FACS, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml