NFKBIA, also called IkBa or MAD-3, is one member of a family of cellular proteins that function to inhibit the <a href=../search_result.php?search_txt=nfkb>NF-kB</a> transcription factor. It inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates <a href=../search_result.php?search_txt=ctnnb1>beta Catenin</a> and IkBa. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, IkBa blocks the ability of NFkB transcription factors to bind to DNA, which is required for proper NFkB functioning.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 3-112) was used as the immunogen for this IkBa antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
IkBa
Gene Id
4792
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the IkBa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml