IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A recombinant rat protein corresponding to amino acids A29-K268 was used as the immunogen for the IGFBP3 antibody.
Isotype
IgG
Predicted Reactivity
Rat
Reactivity
Rat
Recombinant
No
Subcellular Location
Secreted
Antigen
IGFBP3
Uniprot
P15473
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the IGFBP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, Direct ELISA
Dilution
Western Blot: 0.5-1ug/ml,Direct ELISA (recombinant rat protein): 0.1-0.5ug/ml (BSA-free formulation available)