HSF2 Antibody / Heat shock factor 2

Mfr.No.	R30687
Clonality	Polyclonal
Description	Heat shock transcription factor 2 is a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Mouse HSF2 was expressed in all three embryonic layers at embryonic day 7.5 and the head fold was strongly stained at embryonic day 8.5. In adults, expression is detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells. It mediates Hsp70i bookmarking, binding to this promoter in mitotic cells, recruits protein phosphatase-2A, and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. And HSF2-null mice were born at the expected mendelian ratio but had brain abnormalities and meiotic and gametogenic defects in both genders.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	An amino acid sequence from the middle region of human HSF2 (KQERDGPVEFQHPYFKQGQDD) was used as the immunogen for this HSF2 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Mouse, Rat, Human
Recombinant	No

Antigen	HSF2
Uniprot	Q03933

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the HSF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	IHC-P, IF, WB, FACS
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells