Histone acetyltransferase 1, also known as HAT1, is an enzyme that, in humans, is encoded by the HAT1 gene. The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids EKFLVEYKSAVEKKLAEYK from the human protein were used as the immunogen for the Histone acetyltransferase 1 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the Histone acetyltransferase 1 antibody should be determined by the researcher.
Uniprot
O14929
Format
Antigen affinity purified
Purity
Affinity purified
Storage
After reconstitution, the Histone acetyltransferase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
FACS, IF, WB
Description
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells