Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
Gli3
Uniprot
P10071
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Gli3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.