ADP-ribosylation factor-binding protein GGA3 is a protein that in humans is encoded by the GGA3 gene. This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An E. coli-derived human protein (amino acids E7-P535) was used as the immunogen for the GGA3 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the GGA3 antibody should be determined by the researcher.
Uniprot
Q9NZ52
Format
Antigen affinity purified
Purity
Affinity purified
Storage
After reconstitution, the GGA3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, FACS, Direct ELISA
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml