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Home / Primary Antibodies / Polyclonal Antibodies

EIF4G1 Antibody

SKU : BHA17110161

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Mfr.No.	RQ5515
Clonality	Polyclonal
Description	Eukaryotic translation initiation factor 4 gamma 1 is a protein that in humans is encoded by the EIF4G1 gene. It is mapped to 3q27.1. The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Human recombinant proteins (amino acids Q497-R681 + E1409-L1515) were used as the immunogen for the EIF4G1 antibody.
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Rat, Mouse, Human
Recombinant	No

Antigen	EIF4G1
Uniprot	Q04637

Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format	Antigen affinity purified
Purification	Affinity purified
Storage	After reconstitution, the EIF4G1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	Direct ELISA, FACS, IF, IHC-P, WB
Dilution	Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17110161	RQ5515	100ug	$439		Add to Cart

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