E2F-associated phosphoprotein is a protein that in humans is encoded by the EAPP gene. It is mapped to 14q13.1. This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. Alternative splicing results in multiple transcript variants.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids M1-S285) was used as the immunogen for the EAPP antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse
Reactivity
Human, Mouse
Recombinant
No
Subcellular Location
Nuclear, cytoplasmic
Antigen
EAPP
Uniprot
Q56P03
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the EAPP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
Direct ELISA, IHC-P, IF, WB, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml