Cyclin D3 is a protein that in humans is encoded by the CCND3 gene. It is mapped to 6p21.1. The protein encoded by this gene belongs to a family of proteins whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D3 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. In addition, overexpression of CCND3 upregulated the translational activity in HeLa cells in a dose-dependent manner.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 136-292) was used as the immunogen for this Cyclin D3 antibody.
Isotype
IgG
Predicted Reactivity
Human, Rat
Reactivity
Human, Rat
Recombinant
No
Antigen
Cyclin D3
Gene Id
896
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Cyclin D3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
FACS, WB
Dilution
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells