G2/mitotic-specific cyclin-B2 is a protein that in humans is encoded by the CCNB2 gene. This gene is mapped to 15q22.2. Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids K23-A391) was used as the immunogen for the Cyclin B2 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Rat, Mouse, Human
Recombinant
No
Antigen
Cyclin B2
Uniprot
O95067
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the Cyclin B2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
Direct ELISA, FACS, IF, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml