CD3 epsilon is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. Together with CD3gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, CD3e forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the <font face=symbol>e</font>, <font face=symbol>g</font> and <font face=symbol>d</font> polypeptides are located in the same cluster on chromosome 11. The <font face=symbol>e</font>-polypeptide plays an essential role in T-cell development.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 23-207) was used as the immunogen for this CD3 epsilon antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Rat, Mouse, Human
Recombinant
No
Antigen
CD3 epsilon
Gene Id
916
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CD3 epsilon antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IF, ICC, IHC-F, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 1-2ug/ml