CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids F161-L349 from the human protein were used as the immunogen for the CD11a antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
CD11a
Uniprot
P20701
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CD11a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
ICC, FACS, WB
Dilution
Western blot: 0.5-1ug/ml,FACS: 1-2ug/ml,Immunocytochemistry: 1-2ug/ml