SCFR (Mast/stem cell growth factor receptor), also known as CD117, is a protein that in humans is encoded by the KIT gene. c-Kit was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. It is expressed on the surface of germ cells up to the pachytene stage. Signaling from the receptor, tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the c-Kit effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 26-285) was used as the immunogen for this c-Kit antibody. Human protein shares 66% amino acid sequence identity with mouse protein.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Antigen
c-Kit
Gene Id
3815
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the c-Kit antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
FACS, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells