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Home / Primary Antibodies / Polyclonal Antibodies

Aurora B Antibody / AURKB

SKU : BHA17104443

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NSJ Bioreagents

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Mfr.No.	R30904
Clonality	Polyclonal
Description	Aurora kinase B, also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that AURKB is expressed in the S phase and persistently thereafter. Western blot analysis indicated that it is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected Aurora B from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	An amino acid sequence from the N-terminus of mouse AURKB (QNKQPFTIDNFEIGRP) was used as the immunogen for this Aurora B antibody.
Isotype	IgG
Predicted Reactivity	Mouse, Rat
Reactivity	Rat, Mouse
Recombinant	No

Antigen	Aurora B
Uniprot	O70126

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the Aurora B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB
Dilution	Western blot: 0.5-1ug/ml

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17104443-1	R30904	100ug	$439		Add to Cart

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