ATP5H is the 'd' subunit of Mitochondrial ATP synthase complex which catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo has nine known subunits (a, b, c, d, e, f, g, F6 and 8). The ATP5H gene encodes the d subunit of the Fo complex.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 2-161) was used as the immunogen for this ATP5H antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Mouse, Rat, Human
Recombinant
No
Antigen
ATP5H
Gene Id
10476
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the ATP5H antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, ICC, WB, IF
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml