ATP Citrate Lyase Antibody / ACL

Mfr.No.	R32492
Clonality	Polyclonal
Description	ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Amino acids M1-I180 from the human protein were used as the immunogen for the ATP Citrate Lyase antibody.
Isotype	IgG
Predicted Reactivity	Human, Rat
Reactivity	Rat, Human
Recombinant	No
Subcellular Location	Cytoplasmic, nuclear

Antigen	ACL
Uniprot	P53396

Buffer	Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	IHC-P, ICC, WB, FACS
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells