Arginase, type II encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al.(1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al.(1996) showed that ARG2 mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of the protein and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that it is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the N-terminus of human Arginase II (DFGDLSFTPVPKDDLYNNL) was used as the immunogen for this Arg2 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
Arg2
Uniprot
P78540
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Arg2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml