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Home / Primary Antibodies / Polyclonal Antibodies

Anti-VPS4B/MIG1 Antibody Picoband®

SKU : BHA21002705

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Mfr.No.	A03403-1, A03403-1-10ug, A03403-1-Biotin, A03403-1-Cy3, A03403-1-Dylight488, A03403-1-Dylight550, A03403-1-Dylight594, A03403-1-FITC, A03403-1-HRP, A03403-1-APC, A03403-1-PE, A03403-1-iFluor647, A03403-1-carrier-free
Alternative Names	vacuolar protein sorting 4 homolog B
Background	Vacuolar protein sorting-associated protein 4B is a protein that in humans is encoded by the VPS4B gene. It is mapped to 18q21.33. The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-VPS4B/MIG1 Antibody Picoband® catalog # A03403-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the
Host	Rabbit
Immunogen Region	E.coli-derived human VPS4B/MIG1 recombinant protein (Position: M1-K85).
Isotype	Rabbit IgG
Molecular Weight	50 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1
Tissue Specificity	Ubiquitously expressed.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	O75351
Gene Id	9525
Gene Name	VPS4B
Subcellular Location	Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Beyer, A., Scheuring, S., Muller, S., Mincheva, A., Lichter, P., Kohrer, K. Comparative sequence and expression analyses of four mammalian VPS4 genes. Gene 305: 47-59, 2003. <br /><br />2. Feng, Z., Hensley, L., McKnight, K. L., Hu, F., Madden, V., Ping, L., Jeong, S.-H., Walker, C., Lanford, R. E., Lemon, S. M. A pathogenic picoavirus acquires an envelope by hijacking cellular membranes. Nature 496: 367-371, 2013.<br /><br />3. Fujita, H., Umezuki, Y., Imamura, K., Ishikawa, D., Uchimura, S., Nara, A., Yoshimori, T., Hayashizaki, Y., Kawai, J., Ishidoh, K., Tanaka, Y., Himeno, M. Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B. J. Cell Sci. 117: 2997-3009, 2004.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002705-1	A03403-1	-	100 ug	$370		Add to Cart
BHA21002705-2	A03403-1-10ug	Sample	10 ug	$99		Add to Cart
BHA21002705-3	A03403-1-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002705-4	A03403-1-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002705-5	A03403-1-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002705-6	A03403-1-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002705-7	A03403-1-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002705-8	A03403-1-FITC	FITC	100 ug	$570		Add to Cart
BHA21002705-9	A03403-1-HRP	HRP	100 ug	$570		Add to Cart
BHA21002705-10	A03403-1-APC	APC	100 ug	$670		Add to Cart
BHA21002705-11	A03403-1-PE	PE	100 ug	$670		Add to Cart
BHA21002705-12	A03403-1-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002705-13	A03403-1-carrier-free	carrier-free	100 ug	$370		Add to Cart

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