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Home / Primary Antibodies / Polyclonal Antibodies

Anti-TRPV4-ATTO Fluor-550 Antibody

SKU : BHA21300109

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Alomone Labs

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Mfr.No.	ACC-034-AO, ACC-034-AO_50 mcl
Lead Time	1-2 Business Days
Immunogen Species	Rat
Background	TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.Alomone Labs offers a highly specific antibody directed against an intracellular epitope of TRPV4. Anti-TRPV4 Antibody (#ACC-034) can be used in western blot, immunocytochemical, and immunohistochemical applications and recognizes TRPV4 from rat samples.
Clonality	Polyclonal
Description	Anti-TRPV4 Antibody (#ACC-034) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples. Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-TRPV4-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.
Formulation	PBS pH7.4, 1% BSA with 0.05% sodium azide
Host	Rabbit
Immunogen Region	Intracellular, C-terminus
Isotype	Rabbit IgG
Reactivity	Human, Rat, Mouse
Reconstitution	50 ul double distilled water (DDW).
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	OTRPC4, VRL-2, VR-OAC, TRP12
UNSPSC Code	41116161
Note	Homology: Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Negative Control: RIC-001-AO Specifictiy: TRPV4 Is Toxin: No KO-Validated: yes Standard quality control of each lot: Western blot analysis (unlabeled antibody, #ACC-034), and immunohistochemistry (labeled antibody). Antigen preadsorption control: 1 ug peptide per 1 ug antibody

Accession Number	Q9ERZ8
Gene Id	66026
Target	OTRPC4, VRL-2, VR-OAC, TRP12
Sequence	CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4

Conjugates	ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Concentration	1 mg/ml
Form	Lyophilized powder
Preservative	1% BSA, 0.05% NaN3
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	IF, IHC
Dilution	Immunohistochemistry paraffin embedded sections ihc: 1:20 Western blot wb: Contact Biohippo for information

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300109-1	ACC-034-AO_50 mcl	50 mcl	$797		Add to Cart

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