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Home / Primary Antibodies / Polyclonal Antibodies

Anti-SDHA Antibody Picoband®

SKU : BHA21002620

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Mfr.No.	A01753, A01753-10ug, A01753-Biotin, A01753-Cy3, A01753-Dylight488, A01753-Dylight550, A01753-Dylight594, A01753-FITC, A01753-HRP, A01753-APC, A01753-PE, A01753-iFluor647, A01753-carrier-free
Alternative Names	succinate dehydrogenase complex flavoprotein subunit A
Background	Succinate dehydrogenase complex, subunit A, flavoprotein variant is a protein that in humans is encoded by the SDHA gene. It is mapped to 5p15.33. This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alteatively spliced transcript variants encoding different isoforms have been found for this gene.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-SDHA Antibody Picoband® catalog # A01753. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human SDHA, identical to the related mouse and rat sequences.
Isotype	Rabbit IgG
Molecular Weight	73 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Succinate dehydrogenase (ubiquinone) flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II; Fp; SDHA; SDH2; SDHF
Tissue Specificity	Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	P31040
Gene Id	6389
Gene Name	SDHA
Subcellular Location	Mitochondrion inner membrane. Peripheral membrane protein. Matrix side.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, ICC, WB
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human, Mouse, Rat<br>

Reference	1. Alston, C. L., Davison, J. E., Meloni, F., van der Westhuizen, F. H., He, L., Hoig-Do, H.-T., Peet, A. C., Gissen, P., Goffrini, P., Ferrero, I., Wassmer, E., McFarland, R., Taylor, R. W. Recessive germline SDHA and SDHB mutations causing leukodystrophy and isolated mitochondrial complex II deficiency. J. Med. Genet. 49: 569-577, 2012.<br /><br />2. Bourgeron, T., Rustin, P., Chretien, D., Birch-Machin, M., Bourgeois, M., Viegas-Pequignot, E., Munnich, A., Rotig, A. Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency. Nature Genet. 11: 144-149, 1995.<br /><br />3. Buichon, N,, Briere, J.-J., Libe, R., Vescovo, L., Riviere, J., Tissier, F., Jouanno, E., Jeunemaitre, X., Benit, P., Tzagoloff, A., Rustin, P., Bertherat, J., Favier, J., Gimenez-Roqueplo, A.-P. SDHA is a tumor suppressor gene causing paraganglioma. Hum. Molec. Genet. 19: 3011-3020, 2010.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002620-1	A01753	-	100 ug	$370		Add to Cart
BHA21002620-2	A01753-10ug	Sample	10 ug	$99		Add to Cart
BHA21002620-3	A01753-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002620-4	A01753-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002620-5	A01753-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002620-6	A01753-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002620-7	A01753-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002620-8	A01753-FITC	FITC	100 ug	$570		Add to Cart
BHA21002620-9	A01753-HRP	HRP	100 ug	$570		Add to Cart
BHA21002620-10	A01753-APC	APC	100 ug	$670		Add to Cart
BHA21002620-11	A01753-PE	PE	100 ug	$670		Add to Cart
BHA21002620-12	A01753-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002620-13	A01753-carrier-free	carrier-free	100 ug	$370		Add to Cart

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