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Home / Primary Antibodies / Polyclonal Antibodies

Anti-RAD18 Antibody Picoband®

SKU : BHA21003972

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Mfr.No.	A01622-2, A01622-2-10ug, A01622-2-Biotin, A01622-2-Cy3, A01622-2-Dylight488, A01622-2-Dylight550, A01622-2-Dylight594, A01622-2-FITC, A01622-2-HRP, A01622-2-APC, A01622-2-PE, A01622-2-iFluor647, A01622-2-carrier-free
Alternative Names	guanine deaminase
Background	E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-RAD18 Antibody Picoband® catalog # A01622-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. .
Host	Rabbit
Immunogen Region	E.coli-derived human RAD18 recombinant protein (Position: M1-D408).
Isotype	Rabbit IgG
Molecular Weight	75 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Guanine deaminase; Guanase; Guanine aminase; Guanine aminohydrolase; GAH; p51-nedasin; GDA; KIAA1258
Tissue Specificity	Expressed in lung carcinoma cell lines but not in normal lung tissues.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q9NS91
Gene Id	56852
Gene Name	RAD18
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, IHC, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10^6 cells, Human ELISA, 0.1-0.5 ug/ml, -

Reference	1. Adams, D. J., van der Weyden, L., Gergely, F. V., Arends, M. J., Ng, B. L., Tannahill, D., Kanaar, R., Markus, A., Morris, B. J., Bradley, A. BRCTx is a novel, highly conserved RAD18-interacting protein. Molec. Cell. Biol. 25: 779-788, 2005. 2. Branzei, D., Vanoli, F., Foiani, M. SUMOylation regulates Rad18-mediated template switch. Nature 456: 915-920, 2008. 3. Centore, R. C., Yazinski, S. A., Tse, A., Zou, L. Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response. Molec. Cell 46: 625-635, 2012.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003972-1	A01622-2	-	100 ug	$370		Add to Cart
BHA21003972-2	A01622-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21003972-3	A01622-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003972-4	A01622-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003972-5	A01622-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003972-6	A01622-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003972-7	A01622-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003972-8	A01622-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21003972-9	A01622-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21003972-10	A01622-2-APC	APC	100 ug	$670		Add to Cart
BHA21003972-11	A01622-2-PE	PE	100 ug	$670		Add to Cart
BHA21003972-12	A01622-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003972-13	A01622-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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