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Home / Primary Antibodies / Polyclonal Antibodies

Anti-PKC gamma/PRKCG Antibody Picoband®

SKU : BHA21002066

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Mfr.No.	A01890, A01890-10ug, A01890-Biotin, A01890-Cy3, A01890-Dylight488, A01890-Dylight550, A01890-Dylight594, A01890-FITC, A01890-HRP, A01890-APC, A01890-PE, A01890-iFluor647, A01890-carrier-free
Alternative Names	protein kinase C, gamma
Background	The gamma isotype of protein kinase C (PKC gamma) is a member of the classical PKC (cPKC) subfamily which is activated by Ca (2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain. Within the brain, PKC gamma is the most abundant in the cerebellum, hippocampus and cerebral cortex, where the existence of neuronal plasticity has been demonstrated. PKC gamma gene is mutated in spinocerebellar ataxia type 14 (SCA14). Verbeek et al. (2005) point out the specific alterations in mutant PKC gamma function that could lead to the selective neuronal degeneration of SCA14.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PKC gamma/PRKCG Antibody Picoband® catalog # A01890. Tested in ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of recept
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human PKC gamma/PRKCG, identical to the related mouse and rat sequences.
Isotype	Rabbit IgG
Molecular Weight	78 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Protein kinase C gamma type; PKC-gamma; PRKCG; PKCG
Tissue Specificity	Expressed in Purkinje cells of the cerebellar cortex.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	P05129
Gene Id	5582
Gene Name	PRKCG
Subcellular Location	Cell membrane. Peripheral membrane protein. Cytoplasm. perinuclear region. Synaptosome. Dendrite.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 5ug/ml

Reference	1. Saito N, Shirai Y (2003). "Protein kinase C gamma (PKC gamma): function of neuron specific isotype.". J. Biochem. 132 (5): 6837.<br /><br />2. Verbeek, D. S.; Knight, M. A.; Harmison, G. G.; Fischbeck, K. H.; Howell, B. W. : Protein kinase C gamma mutations in spinocerebellar ataxia 14 increase kinase activity and alter membrane targeting. Brain 128: 436-442, 2005.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002066-1	A01890	-	100 ug	$370		Add to Cart
BHA21002066-2	A01890-10ug	Sample	10 ug	$99		Add to Cart
BHA21002066-3	A01890-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002066-4	A01890-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002066-5	A01890-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002066-6	A01890-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002066-7	A01890-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002066-8	A01890-FITC	FITC	100 ug	$570		Add to Cart
BHA21002066-9	A01890-HRP	HRP	100 ug	$570		Add to Cart
BHA21002066-10	A01890-APC	APC	100 ug	$670		Add to Cart
BHA21002066-11	A01890-PE	PE	100 ug	$670		Add to Cart
BHA21002066-12	A01890-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002066-13	A01890-carrier-free	carrier-free	100 ug	$370		Add to Cart

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