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Home / Primary Antibodies / Polyclonal Antibodies

Anti-PHTF1 Antibody Picoband®

SKU : BHA21005221

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Mfr.No.	A12488, A12488-10ug, A12488-Biotin, A12488-Cy3, A12488-Dylight488, A12488-Dylight550, A12488-Dylight594, A12488-FITC, A12488-HRP, A12488-APC, A12488-PE, A12488-iFluor647, A12488-carrier-free
Alternative Names	histone cluster 1 H3 family member a/b/c/d/e/f/g/h/i/j
Background	HTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alteate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PHTF1 Antibody Picoband® catalog # A12488. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic
Host	Rabbit
Immunogen Region	E.coli-derived human PHTF1 recombinant protein (Position: I188-Q430).
Isotype	Rabbit IgG
Molecular Weight	100 kDa
Reactivity	Human
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3FJ
Tissue Specificity	Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q9UMS5
Gene Id	10745
Gene Name	PHTF1
Subcellular Location	Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.

Applications	WB, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5 ug/ml, Human<br> Flow Cytometry (Fixed), 1-3 ug/1x1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 ug/ml, -<br>

Reference	1. Manuel, A., Beaupain, D., Romeo, P. H., Raich, N. Molecular characterization of a novel gene family (PHTF) conserved from Drosophila to mammals. Genomics 64: 216-220, 2000. <br /><br />2. Raich, N., Mattei, M. G., Romeo, P. H., Beaupain, D. PHTF, a novel atypical homeobox gene on chromosome 1p13, is evolutionarily conserved. Genomics 59: 108-109, 1999.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21005221-1	A12488	-	100 ug	$370		Add to Cart
BHA21005221-2	A12488-10ug	Sample	10 ug	$99		Add to Cart
BHA21005221-3	A12488-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21005221-4	A12488-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21005221-5	A12488-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21005221-6	A12488-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21005221-7	A12488-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21005221-8	A12488-FITC	FITC	100 ug	$570		Add to Cart
BHA21005221-9	A12488-HRP	HRP	100 ug	$570		Add to Cart
BHA21005221-10	A12488-APC	APC	100 ug	$670		Add to Cart
BHA21005221-11	A12488-PE	PE	100 ug	$670		Add to Cart
BHA21005221-12	A12488-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21005221-13	A12488-carrier-free	carrier-free	100 ug	$370		Add to Cart

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