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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Phospholipase A2/PLA2G4A Antibody Picoband®

SKU : BHA21007262

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Mfr.No.	PA2047, PA2047-10ug, PA2047-Biotin, PA2047-Cy3, PA2047-Dylight488, PA2047-Dylight550, PA2047-Dylight594, PA2047-FITC, PA2047-HRP, PA2047-APC, PA2047-PE, PA2047-iFluor647, PA2047-carrier-free
Alternative Names	Cytosolic phospholipase A2
Background	PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-ed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue (s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995).
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Phospholipase A2/PLA2G4A Antibody catalog # PA2047. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related rat and mouse sequences by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	58 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Cytosolic phospholipase A2;cPLA2;Phospholipase A2 group IVA;Phospholipase A2;3.1.1.4;Phosphatidylcholine 2-acylhydrolase;Lysophospholipase;3.1.1.5;PLA2G4A;CPLA2, PLA2G4;
Tissue Specificity	Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P47712
Gene Name	PLA2G4A
Sequence	Contains 1 C2 domain.
Subcellular Location	Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Rat, Mouse<br>

Reference	1. Sharp, J. D., Pickard, R. T., Chiou, X. G., Manetta, J. V., Kovacevic, S., Miller, J. R., Varshavsky, A. D., Roberts, E. F., Strifler, B. A., Brems, D. N., Kramer, R. M. Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 269: 23250-23254, 1994. <br /><br />2. Skorecki, K. L. Personal Communication. Toronto, Canada 4/19/1995.<br /><br />3. Tay, A., Simon, J. S., Squire, J., Hamel, K., Jacob, H. J., Skorecki, K. Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 26: 138-141, 1995.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007262-1	PA2047	-	100 ug	$370		Add to Cart
BHA21007262-2	PA2047-10ug	Sample	10 ug	$99		Add to Cart
BHA21007262-3	PA2047-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007262-4	PA2047-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007262-5	PA2047-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007262-6	PA2047-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007262-7	PA2047-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007262-8	PA2047-FITC	FITC	100 ug	$570		Add to Cart
BHA21007262-9	PA2047-HRP	HRP	100 ug	$570		Add to Cart
BHA21007262-10	PA2047-APC	APC	100 ug	$670		Add to Cart
BHA21007262-11	PA2047-PE	PE	100 ug	$670		Add to Cart
BHA21007262-12	PA2047-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007262-13	PA2047-carrier-free	carrier-free	100 ug	$370		Add to Cart

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